This study was undertaken to determine if Salviae Radix-aquacupuncture (SRA) has a protective effect against rhabdomyolysis-induced acute renal failure in rabbits. Rabbits were dehydrated for 24 hr and then injected with 10 ㎖/㎏ of 50 o glycerol, ne-half of the dose in each hindlimb muscle. In experiments for SRA effect, rabbits ceived 0.5 ㎖ of Salviae Radix extraction in the both points corresponding to She｀nshu￣(BL_(23)) for 7 days before the injection of glycerol. Glycerol injection increased serum creatinine and BUN levels, and induced a decrease in urine volume and glomerular filtration rate, which were accompanied by an increase in fractional excretion of Na+, glucose and phosphate. Glycerol injection resulted in a decrease in renal blood flow. These results show that glycerol injection results in acute renal failure. SRA significantly attenuated alterations in renal function induced by glycerol injection. Glycerol injection caused impairment in p-aminohippurate uptake by renal cortical slices and Na-K-ATPase activity in microsomal fraction, which were prevented by SRA. Glycerol injection increased lipid peroxidation in renal cortical and medullar tissues, which were significantly prevented by SRA. Morphological analysis showed that glycerol injection produced tubular obstruction, and such changes were attenuated by SRA.
These results suggest that SRA prevents rhabdomyolysis-induced acute renal failure, and it therefore could be used in prevention and treatment of lipid peroxidaiton-induced acute renal failure. However, the precise mechanisms of SRA protection remain to be determined.
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